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| Registros recuperados: 46 | |
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| Al-Faifi,Sulieman A; Migdadi,Hussein M; Algamdi,Salem S; Altaf Khan,Mohammad; Ammar,Megahed H; Al-Obeed,Rashid S; I. Al-Thamra,Mohammad; EL-Harty,Ehab H; Jakse,Jerenj. |
| Background: The present study was undertaken towards the development of SSR markers and assessing genetic relationships among 32 date palm (Phoenix dactylifera L.) representing common cultivars grown in different geographical regions in Saudi Arabia. Results: Ninety-three novel simple sequence repeat markers were developed and screened for their ability to detect polymorphism in date palm. Around 71% of genomic SSRs were dinucleotide, 25% tri, 3% tetra and 1% penta nucleotide motives. Twenty-two primers generated a total of 91 alleles with a mean of 4.14 alleles per locus and 100% polymorphism percentage. A 0.595 average polymorphic information content and 0.662 primer discrimination power values were recorded. The expected and observed heterozygosities... |
| Tipo: Journal article |
Palavras-chave: Expected and observed heterozygosity; Microsatellite markers; Polymorphic information content; Polymorphism; Primer discrimination power. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300002 |
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| Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann. |
| A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001 |
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| Bhaumik,Pranami; Ghosh,Priyanka; Ghosh,Sujay; Feingold,Eleanor; Ozbek,Umut; Sarkar,Biswanath; Dey,Subrata Kumar. |
| Abstract Alzheimer's disease and Down syndrome often exhibit close association and predictively share common genetic risk-factors. Presenilin-1 (PSEN-1) and Apolipoprotein E (APOE) genes are associated with early and late onset of Alzheimer's disease, respectively. Presenilin −1 is involved in faithful chromosomal segregation. A higher frequency of the APOE ε4 allele has been reported among young mothers giving birth to Down syndrome children. In this study, 170 Down syndrome patients, grouped according to maternal meiotic stage of nondisjunction and maternal age at conception, and their parents were genotyped for PSEN-1 intron-8 and APOE polymorphisms. The control group consisted of 186 mothers of karyotypically normal children. The frequencies of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome; Genetic polymorphism; Karyotype; Meiosis; Microsatellite markers. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400577 |
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| Boudry, Pierre. |
| To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield. In Europe, where both natural and... |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf |
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| Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois. |
| Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each... |
| Tipo: Text |
Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf |
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| Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno. |
| To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective... |
| Tipo: Text |
Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf |
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| Brugnara,Eduardo Cesar; Bastianel,Marinês; Weiler,Roberto Luis; Schwarz,Sergio Francisco. |
| The objective of this work was to confirm the hybrids obtained in plants originated from the crossing between the mandarins Citrus deliciosa 'Montenegrina' and C. nobilis 'King', and to estimate the genetic similarity among hybrids, and between each hybrid and its parents. Twenty‑three pairs of microsatellite primers were tested. Fourteen of these pairs showed polymorphic bands between parents. Primers CCSM 129 and CCSME 52 were sufficient to identify the 12 nucellar clones observed in the studied population. Genetic similarity analysis of the population (hybrids and parents) showed 0.56 average similarity. Besides the 12 clones of 'Montenegrina' identified, 25 hybrids were found of which D18, C32, D06, C05 and D09 are the more similar to 'Montenegrina'. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus deliciosa; Citrus nobilis; Hybrid identification; Microsatellite markers. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800010 |
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| Dobek,A; Gornowicz,E; Moliński,IA; Grajewski,B; Lisowski,M; Szwaczkowski,T. |
| ABSTRACT Traits associated with meat performance are characterized by a complex inheritance model, including the effects of non-allelic interactions. The aim of this study was to estimate the significance of non-allelic interactions between pairs of loci in each of two alternative groups based on four important performance traits of ducks: body weight (BW), carcass yield calculated as the ratio of carcass weight to body weight (CY), breast and leg muscle yield as a fraction of carcass weight (BL-CW), and skin weight and abdominal fat, again as a fraction of carcass weight (SF-CW). The experimental material was based on an F2 cross between two parental lines: A-55 (Polish Peking) and GL-30 (French Peking). In total, 368 individuals were recorded. All of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck; Categorized traits; Entropy analysis; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200301 |
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| Dobek,A; Gornowicz,E; Moliński,K; Grajewski,B; Lisowski,M; Szwaczkowski,T. |
| ABSTRACT The objective of this study was to identify genome regions determining duck meat performance traits with possible small variation. In total, 368 crossbred ducks of F2 generation obtained from two parental lines: Pekin-type ducks of Polish origin (A55) and Pekin-type ducks of French origin (GL-30) were recorded. The following seven traits were analyzed: body weight, breast muscle weight, leg muscle weight, water holding capacity in the breast and leg muscles, and color lightness L* of the breast and leg muscles. All birds (including parental and F1 generations) were genotyped (29 microsatellite markers). Means and coefficients of variation (CV) were calculated for 28 full-sibs (four sires by six dams and one sire by four dams). Number of progeny... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Duck; Meat quality; Microsatellite markers. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300437 |
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| Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa. |
| Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086 |
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| Freitas,Suzane Fonseca; Moreira,Heden Luiz Marques; Souza,Daiane Machado; Moreira,Carla Giovane Ávila; Tavares,Rafael Aldrighi; Dionello,Nelson José Laurino. |
| ABSTRACT This study aimed to develop PCR assays, reaction combinations, and validation of tetranucleotide SSR loci for tilapia, to minimize the impact of erroneous allele inferences on genotype determination of this marker. Microsatellites containing tetranucleotide repeats were obtained from tilapia genome, version 2.1, avoiding loci in the same linking group. Primers were designed for different fragment sizes, and fluorescence added to each locus. A total of 10 loci were amplified, separately and in combination, and loaded into a single capillary sequencer panel. Alleles were amplified without stutters and easily interpreted. PCR amplifications of DNA repeatedly extracted from samples, and genotyping at different PCR rounds were performed to infer allele... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic variability; Genotyping; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902020000400409 |
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| Ganopoulos,Ioannis; Tourvas,Nikolaos; Xanthopoulou,Aliki; Aravanopoulos,Filippos A.; Avramidou,Euaggelia; Zambounis,Antonios; Tsaftaris,Athanasios; Madesis,Panagiotis; Sotiropoulos,Thomas; Koutinas,Nikolaos. |
| ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward’s... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Genetic diversity; Genotyping; Microsatellite markers; Multivariate analysis; Phenotyping. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600509 |
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| Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin. |
| ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100 |
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| Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga. |
| The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium.; Melhoramento Vegetal. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218 |
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| Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga. |
| The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.03.05-9; 2.08.04.00-8 Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium. Melhoramento Vegetal. |
| Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218 |
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| Kuldeep,Tyagi; Nandan,Rajiv; Kumar,Uttam; Prasad,Lal Chand; Chand,Ramesh; Joshi,Arun Kumar. |
| Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and F6-7, the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F6/F6-7) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite markers; Molecular marker; Spot blotch; Cochliobolus sativus; Barley. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400021 |
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| Registros recuperados: 46 | |
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